President Obama takes the top spot for his election night tweet of "four more years" along with a picture of him hugging First Lady Michelle Obama.
It got more than 810,000 retweets and more than 300,000 favorites.
Justin Bieber came in a distant second for his Tweet about a young fan who passed away from a rare form of brain cancer.
Twitter also rounded up the global events that generated the most tweets and retweets.
The Summer Olympics from London generated over 150 million tweets, followed by the first U.S. presidential debate, election day, the Super Bowl and Hurricane Sandy.